領(lǐng)學(xué)術(shù)科研之先，創(chuàng)食品科技之新
—— 中國食品雜志社
Oyster, as a common aquatic food, play an important role in shellfish allergy. In this study, 2 tropomyosin (TM) isoforms TM-α and TM-β (TM-α/-β) in Alectryonella plicatula were identified. The sequences of 852 bp encoding 284 amino acids of TM-α/-β and 2 recombinant proteins were obtained, respectively. There were 12 amino acid differences between TM-α/-β. The results of immunological experiments indicated that TM-β had stronger immunobinding activity and immunoreactivity than those of TM-α. Structural analysis showed that TM-β had more α-helix and higher surface hydrophobicity than TM-α. Sequences and epitopes alignment with shellfish TMs revealed that amino acids of TM-β were more frequently recognized as IgE epitopes in other shellfish TMs than TM-α. Differences in structure and sequence account for the higher immunological activity of TM-β compared to TM-α. These findings provide a theoretical basis for enriching the understanding of shellfish TM and accurate diagnosis of allergic components.
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