The present study was conducted to evaluate the hypolipidemic effects of coffee silver skin (CSS) supplementation

in rats fed a highfat diet (HFD). A total of 40 albino rats were used in the present study.

The groups were as follows: Rats fed a normal diet, N group; high fat diet, HFD group; high fat diet +10%

CSS, HFD 10; HFD+ 15% CSS, HFD 15; HFD+ 20% CSS, HFD 20; the diets were followed for 8 weeks. Blood

samples were collected at the end of the experiment. At the time of sacrifice, the weights of heart, liver,

kidneys, epididymal fat and retroperitoneal fat of the experimental rats with respect to body weight were

recorded. The lipid parameter of the serum was recorded and liver and kidney function tests were conducted.

Finally, a histopathological assay was performed on the liver and kidney tissues of the rats fed the

tested diets. The weight gain of the rats fed a HFD supplemented with 10, 15 and 20% CSS was ～1.05, 1.08

and 1.12 times lower than that of those rats fed HFD, respectively. The incorporation of CSS at a level of 20%

reduced the increase in liver, kidney, epididymal fat and retroperitoneal fat weight by 17.84, 19.38, 47.23

and 18.00%, respectively, compared with HFD alone. HFD administration induced considerable increases

in alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities compared

with the control group. The results also indicated that the HFD-fed rats exhibited increased levels of urea,

uric acid and creatinine, by ～26.38, 8.40 and 6.75%, respectively, compared with the control rats. With

the exception of high-density lipoprotein cholesterol, all lipid fractions increased significantly in rats fed

a HFD. The administration of a HFD induced marked pathological changes in the liver and kidneys of the

rats. However, the incorporation of various levels of CSS in to a HFD reduced these changes. The results

of the present study illustrate that the incorporation of CSS into HFDs reduces the hyperlipidemia effect

of these diets.