領(lǐng)學(xué)術(shù)科研之先，創(chuàng)食品科技之新
—— 中國(guó)食品雜志社
A rapid method was presented for the determination of 19 polyphenols in tea by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS). Tea samples were extracted by 50% (V/V) ethanol, then separated by Waters Acquity BEH C18?column using a binary solvent system composed of?acetonitrile?and water (0.1% formic acid) by?gradient elution. The analytes were determined by Q-TOF MS in TOF MS and information dependent acquisition (IDA)-MS/MS mode. The results showed that mass accuracy error of the 19 polyphenols were lower than 5.0 × 10–6, good linear relationship was got in range of 0.2–500 μg/L and?correlation coefficient?was higher than 0.9990. The?LOD?was in the range of 0.002–0.100 mg/kg and the?LOQ?was in the range of 0.004–0.200 mg/kg. Recovery of the method was in range of 78.4%–109.2% with spike levels of 0.004–2.000 mg/kg, relative standard deviations were lower than 10%. The method was simple, rapid and accurate. It could be used for the rapid screening and?quantitative analysis?of 19 polyphenols in tea.
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