領(lǐng)學(xué)術(shù)科研之先，創(chuàng)食品科技之新
—— 中國(guó)食品雜志社
Two immunomodulatory?polysaccharides?(Vp2a-II and Vp3) were isolated and identified from?Apocynum?venetum?L. flowers, and their innate immune-stimulating functions and working mechanisms were evaluated in RAW264.7 cells. Both the level of released?nitric oxide?(NO) and expression of?inducible nitric oxide synthase?(iNOS) mRNA were significantly enhanced in the RAW264.7 macrophages cells treated by Vp2a-II and Vp3. Vp2a-II (100–800 μg/mL) and Vp3 (400 μg/mL) could significantly increase the?phagocytic activity?of RAW264.7 cells and the secretion and mRNA expression of?TNF-α?and?IL-6?in a concentration-dependent manner through affecting mitogen-activated protein kinase (MAPK) activity and nuclear factor κB (NF-κB) nuclear translocation. Vp2a-II might activate the MAPK?signaling pathways?and induce the nuclear translocation of NF-κB p65, whilst Vp3 likely activated the NF-κB and MAPK signaling pathways without influencing the?p38 MAPK?route.
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