領(lǐng)學(xué)術(shù)科研之先,創(chuàng)食品科技之新
—— 中國食品雜志社
期刊集群
Structural characteristics, anticoagulant and antithrombotic mechanism of a novel polysaccharide from Rosa Chinensis Flos
來源:食品科學(xué)網(wǎng) 閱讀量: 126 發(fā)表時間: 2022-10-28
作者: Xiaofeng Zhang, Zhenhua Liang, Geoffrey Ivan Neil Waterhouse, Shengjun Jiang, Dongxiao Sun-Waterhouse, Jinmei Wang, Changyang Ma, Wenyi Kang
關(guān)鍵詞: Rosa Chinensis Flos; Polysaccharide isolation; Antithrombotic activity; Intestinal flora
摘要:

This is the first report on a polysaccharide (RCJ2-Ib) isolated from Rosa Chinensis Flos. RCJ2-Ib was obtained through the extraction with water, precipitation with ethanol, separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column. GC, FT-IR and NMR analyses revealed that RCJ2-Ib (3.3 kDa) was a 1,4-linked polymannuronic acid containing substantial β-D-anomers units. The anticoagulant effect of RCJ2-Ib evaluated by using rabbit ear venous blood and an acute blood stasis rat model showed that RCJ2-Ib had obvious anticoagulant activity in regulating endogenous and exogenous coagulation pathways and reducing serum thromboxane B2 and endothelin-1. In addition, RCJ2-Ib could also increase the number of Lactobacillus and Escherichia coli. As a result, RCJ2-Ib has the potential to inhibit thrombosis and maintain the intestinal environment.

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